Growth of Engineered Pseudomonas putida KT2440 on Glucose, Xylose and Arabinose: Hemicellulose Hydrolysates and Their Major Sugars as Sustainable Carbon Sources

Feedstocks, Germany, Process, R & D Focus, Sugars, University/College Programs

February 5, 2019

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by Yan Wang, Felix Horlamus, Marius Henkel, Filip Kovacic, Sandra Schläfle, Rudolf Hausmann, Andreas Wittgens, Frank Rosenau (Global Change Biology Bioenergy) Lignocellulosic biomass is the most abundant bioresource on earth containing polymers mainly consisting of D‐glucose, D‐xylose, L‐arabinose and further sugars. In order to establish this alternative feedstock apart from applications in food, we engineered Pseudomonas putida KT2440 as microbial biocatalyst for the utilization of xylose and arabinose in addition to glucose as sole carbon sources. The D‐xylose metabolizing strain P. putida KT2440_xylAB and L‐arabinose metabolizing strain P. putida KT2440_araBADwere constructed by introducing respective operons from Escherichia coli. Surprisingly, we found out that both recombinant strains were able to grow on xylose as well as arabinose with high cell densities and growth rates comparable to glucose. In addition, the growth characteristics on various mixtures of glucose, xylose and arabinose were investigated, which demonstrated the efficient co‐utilization of hexose and pentose sugars. Finally, the possibility of using lignocellulose hydrolysate as substrate for the two recombinant strains was verified. The recombinant P. putida KT2440 strains presented here as flexible microbial biocatalysts to convert lignocellulosic sugars will undoubtedly contribute to the economic feasibility of the production of valuable compounds derived from renewable feedstock. READ MORE

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