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Home » Feedstocks, Field/Orchard/Plantation Crops/Residues, India, Process, R & D Focus, Sustainability, University/College Programs

Genetically Engineered Fungus Now Squeezes out More Biofuel from Farm Waste

Submitted by on February 5, 2018 – 11:27 amNo Comment

T V Jayannew (The Hindu Business Line)  India’s efforts to enhance biofuel production may get a boost from a work being done at a research laboratory in New Delhi.

Researchers at the International Centre for Genetic Engineering and Biotechnology (ICGEB) have altered the genome of a commercially-exploited fungus in such a way that it would increase the production of enzymes helpful in converting cellulose into simple fermentable sugars.

The significance of the work lies in the fact that it would help produce double the amount of biofuel from agricultural waste materials, including rice and wheat straw, whose burning was implicated in the worsening of air quality in most parts of northern India. Available estimates show that India produces around 500 million tonnes of agricultural waste annually.

Around 80 per cent of dry biomass – including that of crop residues – contain cellulose and hemocellulose. “Processes currently used for biological conversion of cellulosic biomass are capable of converting only 60-65 per cent of cellulose material into sugars. Our process could enhance the recovery to 80-85 per cent,” the ICGEB scientist said.

The scientists, funded by a research grant from the Department of Biotechnology, have been working the PF fungus since 2009. They opted for this fungus as it was found to produce five times more active enzyme (known as CBH1) in breaking down cellulose as compared to its fungal cousin Trichoderma reesei, more commonly used in industrial enzyme cocktails.

In this work, the ICGEB scientists went a step further and tweaked the PF fungal genome in such a way that the enzyme production can be doubled.   READ MORE  Abstract (Biotechnology for Biofuels)

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