To examine this further, a multi-institutional team of researchers led by scientists from the U.S. Department of Energy’s Joint BioEnergy Institute (JBEI) combined comparative proteomics with biochemical measurements and then assessed differences in glycoside hydrolases produced by diverse microbes in communities cultivated from green waste compost and grown on crystalline cellulose.
Using several mass spectrometry instruments and high-throughput DNA sequencing technologies, the team discovered that a glycoside hydrolase family 12 enzyme produced by the bacterium Thermobispora bispora plays an underappreciated role in breaking down crystalline cellulose.
These findings suggest that the enzyme could be effective at converting plant biomass to fuels and chemicals. The study also illustrates the power of comparative community proteomics to reveal novel insights into microbial enzymes. READ MORE
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